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mad2 proteins blots  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mad2 proteins blots
    Acute loss of Rb leads to chromosome instability and increases MPM2 positive cells . ( A ) pRb deficient MEFs show increased aneuploid metaphases both hypodiploid (<40 chromosomes) and hyperdiploid (>40 chromosomes). Each bar indicates mean + s.e. of three independent experiments. ( B ) pRb deficient MEFs have increased percentage of cells with micronuclei. An average of 100 cells were analyzed for each time point, each bar represents mean + s.e. of two experiments. ( C ) MEFs acutely depleted of pRb (Rb-/-) have increased number of mitotic cells with multiple centrosomes (arrowheads). Mitotic cells and centrosomes were revealed by using primary antibodies against MPM2 (Mitotic Protein Monoclonal) and γ-tubulin, that were detected by FITC (green) and TRITC (red) conjugated secondary antibody respectively. ( D ) Histograms indicating the percentage of MPM2 positive cells in pRb competent and deficient MEFs. An average of 100 cells were analyzed for each time point, each bar represents mean + s.e. of at least two experiments. ( E ) Western blot analysis showing the amount of <t>MAD2</t> protein after 3 days from the adenoviral infection: Rb LoxP/LoxP MEF uninfected ( lane 1), infected with the empty adenoviral vector ( lane 2) and with the Ad-Cre vector ( lane 3).
    Mad2 Proteins Blots, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mad2+proteins+blots/pmc01613254-150-6-23?v=Santa+Cruz+Biotechnology
    Average 94 stars, based on 218 article reviews
    mad2 proteins blots - by Bioz Stars, 2026-07
    94/100 stars

    Images

    1) Product Images from "RB acute loss induces centrosome amplification and aneuploidy in murine primary fibroblasts"

    Article Title: RB acute loss induces centrosome amplification and aneuploidy in murine primary fibroblasts

    Journal: Molecular Cancer

    doi: 10.1186/1476-4598-5-38

    Acute loss of Rb leads to chromosome instability and increases MPM2 positive cells . ( A ) pRb deficient MEFs show increased aneuploid metaphases both hypodiploid (<40 chromosomes) and hyperdiploid (>40 chromosomes). Each bar indicates mean + s.e. of three independent experiments. ( B ) pRb deficient MEFs have increased percentage of cells with micronuclei. An average of 100 cells were analyzed for each time point, each bar represents mean + s.e. of two experiments. ( C ) MEFs acutely depleted of pRb (Rb-/-) have increased number of mitotic cells with multiple centrosomes (arrowheads). Mitotic cells and centrosomes were revealed by using primary antibodies against MPM2 (Mitotic Protein Monoclonal) and γ-tubulin, that were detected by FITC (green) and TRITC (red) conjugated secondary antibody respectively. ( D ) Histograms indicating the percentage of MPM2 positive cells in pRb competent and deficient MEFs. An average of 100 cells were analyzed for each time point, each bar represents mean + s.e. of at least two experiments. ( E ) Western blot analysis showing the amount of MAD2 protein after 3 days from the adenoviral infection: Rb LoxP/LoxP MEF uninfected ( lane 1), infected with the empty adenoviral vector ( lane 2) and with the Ad-Cre vector ( lane 3).
    Figure Legend Snippet: Acute loss of Rb leads to chromosome instability and increases MPM2 positive cells . ( A ) pRb deficient MEFs show increased aneuploid metaphases both hypodiploid (<40 chromosomes) and hyperdiploid (>40 chromosomes). Each bar indicates mean + s.e. of three independent experiments. ( B ) pRb deficient MEFs have increased percentage of cells with micronuclei. An average of 100 cells were analyzed for each time point, each bar represents mean + s.e. of two experiments. ( C ) MEFs acutely depleted of pRb (Rb-/-) have increased number of mitotic cells with multiple centrosomes (arrowheads). Mitotic cells and centrosomes were revealed by using primary antibodies against MPM2 (Mitotic Protein Monoclonal) and γ-tubulin, that were detected by FITC (green) and TRITC (red) conjugated secondary antibody respectively. ( D ) Histograms indicating the percentage of MPM2 positive cells in pRb competent and deficient MEFs. An average of 100 cells were analyzed for each time point, each bar represents mean + s.e. of at least two experiments. ( E ) Western blot analysis showing the amount of MAD2 protein after 3 days from the adenoviral infection: Rb LoxP/LoxP MEF uninfected ( lane 1), infected with the empty adenoviral vector ( lane 2) and with the Ad-Cre vector ( lane 3).

    Techniques Used: Western Blot, Infection, Plasmid Preparation

    Changes in transcript levels of genes associated with mitotic progression after pRb acute loss
    Figure Legend Snippet: Changes in transcript levels of genes associated with mitotic progression after pRb acute loss

    Techniques Used: Binding Assay, Transformation Assay



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    mad2 proteins blots  (Santa Cruz Biotechnology)
    94
    Santa Cruz Biotechnology mad2 proteins blots
    Acute loss of Rb leads to chromosome instability and increases MPM2 positive cells . ( A ) pRb deficient MEFs show increased aneuploid metaphases both hypodiploid (<40 chromosomes) and hyperdiploid (>40 chromosomes). Each bar indicates mean + s.e. of three independent experiments. ( B ) pRb deficient MEFs have increased percentage of cells with micronuclei. An average of 100 cells were analyzed for each time point, each bar represents mean + s.e. of two experiments. ( C ) MEFs acutely depleted of pRb (Rb-/-) have increased number of mitotic cells with multiple centrosomes (arrowheads). Mitotic cells and centrosomes were revealed by using primary antibodies against MPM2 (Mitotic Protein Monoclonal) and γ-tubulin, that were detected by FITC (green) and TRITC (red) conjugated secondary antibody respectively. ( D ) Histograms indicating the percentage of MPM2 positive cells in pRb competent and deficient MEFs. An average of 100 cells were analyzed for each time point, each bar represents mean + s.e. of at least two experiments. ( E ) Western blot analysis showing the amount of <t>MAD2</t> protein after 3 days from the adenoviral infection: Rb LoxP/LoxP MEF uninfected ( lane 1), infected with the empty adenoviral vector ( lane 2) and with the Ad-Cre vector ( lane 3).
    Mad2 Proteins Blots, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mad2+proteins+blots/pmc01613254-150-6-23?v=Santa+Cruz+Biotechnology
    Average 94 stars, based on 1 article reviews
    mad2 proteins blots - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Acute loss of Rb leads to chromosome instability and increases MPM2 positive cells . ( A ) pRb deficient MEFs show increased aneuploid metaphases both hypodiploid (<40 chromosomes) and hyperdiploid (>40 chromosomes). Each bar indicates mean + s.e. of three independent experiments. ( B ) pRb deficient MEFs have increased percentage of cells with micronuclei. An average of 100 cells were analyzed for each time point, each bar represents mean + s.e. of two experiments. ( C ) MEFs acutely depleted of pRb (Rb-/-) have increased number of mitotic cells with multiple centrosomes (arrowheads). Mitotic cells and centrosomes were revealed by using primary antibodies against MPM2 (Mitotic Protein Monoclonal) and γ-tubulin, that were detected by FITC (green) and TRITC (red) conjugated secondary antibody respectively. ( D ) Histograms indicating the percentage of MPM2 positive cells in pRb competent and deficient MEFs. An average of 100 cells were analyzed for each time point, each bar represents mean + s.e. of at least two experiments. ( E ) Western blot analysis showing the amount of MAD2 protein after 3 days from the adenoviral infection: Rb LoxP/LoxP MEF uninfected ( lane 1), infected with the empty adenoviral vector ( lane 2) and with the Ad-Cre vector ( lane 3).

    Journal: Molecular Cancer

    Article Title: RB acute loss induces centrosome amplification and aneuploidy in murine primary fibroblasts

    doi: 10.1186/1476-4598-5-38

    Figure Lengend Snippet: Acute loss of Rb leads to chromosome instability and increases MPM2 positive cells . ( A ) pRb deficient MEFs show increased aneuploid metaphases both hypodiploid (<40 chromosomes) and hyperdiploid (>40 chromosomes). Each bar indicates mean + s.e. of three independent experiments. ( B ) pRb deficient MEFs have increased percentage of cells with micronuclei. An average of 100 cells were analyzed for each time point, each bar represents mean + s.e. of two experiments. ( C ) MEFs acutely depleted of pRb (Rb-/-) have increased number of mitotic cells with multiple centrosomes (arrowheads). Mitotic cells and centrosomes were revealed by using primary antibodies against MPM2 (Mitotic Protein Monoclonal) and γ-tubulin, that were detected by FITC (green) and TRITC (red) conjugated secondary antibody respectively. ( D ) Histograms indicating the percentage of MPM2 positive cells in pRb competent and deficient MEFs. An average of 100 cells were analyzed for each time point, each bar represents mean + s.e. of at least two experiments. ( E ) Western blot analysis showing the amount of MAD2 protein after 3 days from the adenoviral infection: Rb LoxP/LoxP MEF uninfected ( lane 1), infected with the empty adenoviral vector ( lane 2) and with the Ad-Cre vector ( lane 3).

    Article Snippet: To detect amount of pRb and MAD2 proteins blots were probed with mouse monoclonal antibodies anti pRb (554136, Becton Dickinson) and anti MAD2 (Santa Cruz Biotechnology) respectively as recommended by manufacturer.

    Techniques: Western Blot, Infection, Plasmid Preparation

    Changes in transcript levels of genes associated with mitotic progression after pRb acute loss

    Journal: Molecular Cancer

    Article Title: RB acute loss induces centrosome amplification and aneuploidy in murine primary fibroblasts

    doi: 10.1186/1476-4598-5-38

    Figure Lengend Snippet: Changes in transcript levels of genes associated with mitotic progression after pRb acute loss

    Article Snippet: To detect amount of pRb and MAD2 proteins blots were probed with mouse monoclonal antibodies anti pRb (554136, Becton Dickinson) and anti MAD2 (Santa Cruz Biotechnology) respectively as recommended by manufacturer.

    Techniques: Binding Assay, Transformation Assay